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N9528
Sigma
Anti-Neuropeptide Y (NPY) antibody produced in rabbit
whole antiserum
| MDL number: | MFCD00162717 |
Description
| Immunogen | synthetic neuropeptide Y (NPY, porcine), conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY. |
| Specificity | The antibody recognizes by ELISA, NPY (human), NPY (porcine), NPY (sheep), NPY (13-36), NPY (18-36), and cross-reacts with PYY (human). It does not cross-react with neurokinin B (NKB), neurokinin A (NKA), substance P (SP), calcitonin, somatostatin and BSA. |
Properties
| antibody form | whole antiserum |
| clone | (polyclonal) |
| contains | 15 mM sodium azide |
| species reactivity | rat, pig, human, sheep |
| application(s) | immunohistochemistry (frozen sections): 1:8,000-1:16,000 using 4% paraformaldehyde perfusion-fixed sections of rat brain |
| indirect ELISA: 1:5,000-1:10,000 using using peptide coating at 0.5-1 μg/well in 0.05 M carbonate/bicarbonate buffer, pH 9.6, and pNPP as substrate | |
| Gene Information | rat ... Npy(24604) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Franco, A.J., et al., Morphometric and immunohistochemical study of the rumen of red deer during prenatal development. J. Anat. 204, 501-513, (2004) Abstract |
| Franco, A.J., et al., Morphometric and immunohistochemical study of the reticulum of red deer during prenatal development J. Anat. 205, 277-289, (2004) Abstract | |
| Franco, A., et al., Morphometric and immunohistochemical study of the omasum of red deer during prenatal development J. Anat. 206, 543-555, (2005) Abstract |
